| 1. | The monopartite genome of tobacco mosaic virus is a plus-strand rna molecule of 6395 nucleotides .
烟草花叶病毒的单份基因组是一个6395个核甙的（）链RNA分子。 |
| 2. | Compared studies of resistance to tobacco mosaic virus among tobacco varieties
抗性差异的比较研究 |
| 3. | Tobacco mosaic virus
烟草镶嵌病毒 |
| 4. | Spread characteristics of tobacco mosaic virus through clipping in tobacco floating - seedling system
烟草漂浮育苗剪叶传播烟草花叶病毒的特点 |
| 5. | The monopartite genome of tobacco mosaic virus is a plus - strand rna molecule of 6395 nucleotides
烟草花叶病毒的单份基因组是一个6395个核甙的（ ）链rna分子。 |
| 6. | Abstract : tobacco mosaic virus plays an very important role in the discovery of virus and several pioneers made contributions to the developmental process of virology , through which the brief history of discovery of virus can be traced
文摘:烟草花叶病病毒在病毒的发现中有着极其重要的地位，几位病毒学研究的先驱在这方面的研究有极大的贡献，以此为线索可以看到病毒被发现的简要历史。 |
| 7. | An antisense molecular beacon ( mbl ) was designed for the dctonninon of the genome rna of tobacco mosaic virus with no need of stringen isolation and purification of the virus prtc1es and rna as well , which benefited from the proininen specificity and selectivity of molecular beacon
凝g胶中的存在状态，发现酶分子在溶胶凝胶中象在水溶液中一样均匀分布，且不影响活k性。辣根过氧化物酶（ hrp ）和葡萄糖氧化酶（ god ）都能成功地包埋于其中。 |
| 8. | However , only a few host factors with clear in vivo function have been identified . by using pcr and 5 " and 3 " race , we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene , tom1 , which supports the replication of tobacco mosaic virus
根据从拟南芥中已经克隆的支持烟草花叶病毒复制的基因tom1序列设计一对特异性引物，用rt - pcr的方法获得番茄同源基因的部分序列，然后利用5 ’ race与3 ’ race方法从番茄中克隆出全长ttom1基因。 |
| 9. | The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell
第三章，根据一种常见的病毒烟草花叶病毒( tmv )的核酸序列设计了分子信标荧光探针，由于tmv的遗传物质是rna ，分子信标又具有很高的特异性和灵敏度，因此感染了病毒粒子的植物叶片在经过简单处理后，可用分子信标检测叶片上。 |
| 10. | Ih ped one , a nove1 mo1ecuar probe onolecuar beason ( mb ) was developed to establish a stwle and precise method for the quanitative analysis of gentic materials such as ing1 inrn from differen cells and rna fonn tobacco mosaic virus . the milization method for mb was studied for the fabrication of dna biosensor and dna chip . ih pall two , a series of inunobilization method such as sol - gel method and ligh polererization method were investigaed for the otilization of bio - molecues and fluorescence dye
本论文各章内容可归纳如下：第一章，根据抑癌基因ing1基因的序列设计并合成了检测ing1转录产物的分子信标( 5 ' - - tamra - c6 - nh - cgttgatggttccacttctcgtgcgttcaacg - dabcyl - 3 ' ) ，其中tamra代表四甲基罗丹明，为荧光基团， dabcyl代表4 - (对二甲氨基偶氮苯)苯甲酸，为荧光熄灭基团。 |
